| Regulatory reference: | Guidelines to Commission Regulation (EU) No 655/2013 |
| Claim: | Firming efficacy, Improves skin tone and smoothness and Improves skin elasticity |
| Application field: | Visage and body products. |
| Description of the test: | Measurement of R0 and R2 parameters on 20 healthy volunteers. Instrument: MPA580 Cutometer® for the scientific quantification of the viscoelasticity of the skin. Manufacturer: Courage-Khazaka GmbH (Germany). |
| Regulatory reference: | Proprietary method |
| Claim: | Activates the synthesis of collagen, elastin, fibronectin |
| Application field: | Finished products and active ingredients. |
| Description of the test: | Real time PCR analysis of the expression of collagen and fibronectin, compared to an untreated control, to determine the efficacy of a product or ingredient in activating the synthesis of these proteins involved in maintaining skin elasticity and prevent aging. |
| Regulatory reference: | Proprietary method |
| Claim: | Activates the synthesis of collagen, elastin, fibronectin |
| Application field: | Finished products and active ingredients. |
| Description of the test: | Quantitative analysis of the effect of the tested product in increasing the synthesis of collagen and elastin. The test is performed on several concentration and time points using real time PCR on cultured cell lines. The test can be performed on human keratinocytes or fibroblasts. |
| Regulatory reference: | Proprietary method |
| Claim: | Anti-aging and Redensifies and tones the epidermis |
| Application field: | Finished products and active ingredients. |
| Description of the test: | Quantitative evaluation of the efficacy of a product or active ingredient in increasing cell proliferation and protein synthesis. MTT test and protein quantification at different time intervals. The test can be performed on human keratinocytes or fibroblasts. |