| Regulatory reference: | Proprietary method |
| Claim: | Antioxidant and Anti-pollution |
| Application field: | Raw materials and finished products. |
| Description of the test: | In vitro test for the evaluation of the protective effect against air pollution. The purpose of this test is to evaluate the efficacy of the sample in reducing ROS release in response to a mix of heavy metals and atmospheric dust particulate in a skin-derived cell model. |
| Regulatory reference: | OECD n° 492 |
| Claim: | Non-irritant for the eyes |
| Application field: | Chemical substances or mixtures. |
| Description of the test: | Identification of chemical substances or mixtures that do not require classification as (severe) irritants through evaluation of their cytotoxic effects on an in vitro reconstructed corneal epithelium model. NOTE: if the sample qualifies as irritant, further tests are required in order to determine the degree of irritant action. |
| Regulatory reference: | Guidelines to Commission Regulation (EU) No 655/2013 |
| Claim: | Ophthalmologically tested |
| Application field: | Eye contour cosmetics or products that require to confirm the compatibility with the periocular mucosa in normal usage conditions. |
| Description of the test: | An ophthalmology specialist will inspect the target area, and especially the eyelids, before the application using a fissure lamp, in order to confirm the absence of swelling, hyperemia, dryness or desquamation. The inspection also includes any alterations of the eyelashes. In the eye itself, the integrity of the cornea and conjunctiva, the absence of hyperemia, and signs of conjunctival or tarsal irritation are verified. Finally, the absence of qualitative and quantitative alterations in the tear film. The specialist then evaluates and scores erythemal response, tearing, edema and dryness after the product has been used for 30 minutes, 2 days and 30 days to quantify possible alterations induced by the sample. The volunteers also provide subjective evaluations of itching, burning, stinging or foreign body response feeling, and/or sight clouding, at the same endpoints, using the VAS (Visual Analogical Scale) score system. |
| Regulatory reference: | Guidelines to Commission Regulation (EU) No 655/2013 |
| Claim: | Long lasting |
| Application field: | Makeup and lipsticks. |
| Description of the test: | Instrumental colorimetric evaluation: the evaluation can only be performed on pigmented products and is not applicable to transparent cosmetics. Clinical evaluation: digital imaging of the treated area at T=0 and after X hours from the application of the product. The duration of the treatment is agreed upon with the sponsor before the test. |
| Regulatory reference: | Guidelines to Commission Regulation (EU) No 655/2013 |
| Claim: | No transfer |
| Application field: | Makeup and lipsticks. |
| Description of the test: | Digital images of the treated area are captured immediately after application of the product and after contact with test items. Depending on the type of product testing, contact items include skin, paper cups, glass cups, napkins, white clothes. |
| Regulatory reference: | According to current regulations |
| Claim: | Titer of substances, Sensitizing metals below risk threshold and Cadmium, Cobalt, Chrome, Nickel free |
| Description of the test: | Cosmetic products. |
| Description of the test: | Mineralization/high pressure microwave dissolution and determination through ICP-OES. |
| Regulatory reference: | OECD n° 491 |
| Claim: | Non-irritant for the eyes (CLP Labelling) |
| Application field: | Raw ingredients or finished products. |
| Description of the test: | Short term exposure analysis by application of each tested substance on SIRC (Statens Seruminstitut Rabbit Cornea) cells for the determination of the effect on cell viability through MTT test. |
| Regulatory reference: | OECD n° 439 |
| Claim: | Non-irritant for the skin |
| Application field: | Chemical substances or mixtures. |
| Description of the test: | Evaluation of the irritation potential of chemical substances or mixtures through evaluation of their cytotoxic effect on an in vitro reconstructed model of human skin. |
| Regulatory reference: | Proprietary method |
| Claim: | Efficacy of preservatives |
| Application field: | Cosmetic products. |
| Description of the test: | In vitro and in vivo evaluation RDV for the determination of the PAO (Period After Opening). |
| Regulatory reference: | ISO 29621 |
| Claim: | Free water percentage calculation and Low microbial contamination risk |
| Application field: | Cosmetics. |
| Description of the test: | Evaluation of the risk of microbial contamination in a cosmetic product by ISO 29621 method for the determination of water activity (Aw) parameter. |