Skin whitening Archives – abich.ca - Biological and chemical analysis

Regulatory reference:	Proprietary method
Claim:	Skin whitening and Depigmenting action
Application field:	Active ingredients or finished products.
Description of the test:	Quantification of the inhibition of melanin synthesis in human melanoma cells exposed to the substance or product to be tested, in comparison with untreated controls. The same test can also be used to quantify an increase of melanin synthesis.

Regulatory reference:	Guidelines to Commission Regulation (EU) No 655/2013
Claim:	Reduces skin blemishes, Skin whitening and Depigmenting action
Application field:	Visage and body cosmetic products.
Description of the test:	Instrumental measurements performed using a Minolta CR200 colorimeter will be performed at T=0 and T=X days (to be defined by previous agreement with the sponsor) in order to evaluate changes in the pigmentation of the treated area. The measurements will be performed at each time point in the same area. Acquisition of high definition images will be performed at T=0 and T=X days using a macro objective with color normalization and coaxial circular flash.

Regulatory reference:	Proprietary method
Claim:	Skin whitening and Depigmenting action
Application field:	Active ingredients or finished products.
Description of the test:	Evaluation of the changes in the expression of the tyrosinase gene in human melanoma cells.

Skin whitening