| Regulatory reference: | OECD 442D |
| Claim: | No-skin sensitizer and Hypoallergenic |
| Application field: | Substances or mixtures. |
| Description of the test: | The ARE-Nrf2 method employs cytofluorometry to quantify the antioxidant response in a culture of human keratinocytes after exposure to the product. |
| Regulatory reference: | OECD 442E |
| Claim: | No-skin sensitizer and Hypoallergenic |
| Application field: | Substances or mixtures. |
| Description of the test: | H-CLAT cytometry method to evaluate possible changes in the expression of cell surface markers (CD86 e CD54) in cultures of THP-1 human monocytes to exclude the related sensitization pathway. |
| Regulatory reference: | OECD 442C |
| Claim: | No-skin sensitizer and Hypoallergenic |
| Application field: | Substances or mixtures without metal compounds. |
| Description of the test: | The DPRA methods chemically quantifies (by LC-MS/MS or HPLC-UV) the covalent bonds of electrophilic chemical substances (oxidants, electron acceptors) to nucleophilic substances, as known to occur between allergens and skin proteins, in order to exclude the product’s activity in the related sensitization pathway. |