Barrier effect test on 3D reconstructed skin by analysis of the transcutaneous passage of caffeine

Regulatory reference:	Proprietary method
Claim:	Barrier effect
Application field:	Solutions or finished products.
Description of the test:	Quantification by HPLC of the amount of caffeine permeating the skin barrier of in vitro 3D skin in Franz’s cells, compared to the total titer of the same substance in the applied product.